The Food Safety and Standards Authority of India on 13th July 2020 has issued methods of analysis for fortification in food and method of analysis for formulated supplements for children.
The method of determining folic acid in fortified rice and wheat is used as an immunoaffinity folic acid kit which needs to be stored at 4°C and the Folic acid is extracted from the sample using pancreatic and L-ascorbic acid. After incubation, the extract is filtered, diluted with water, and applied to an immuno-affinity column (IAC) containing antibodies specific to folic acid. Folic acid is quantified by reversed-phase liquid chromatography (RP-HPLC) with UV detection.
The Method is for determination of Zinc in fortified wheat & rice flour using ICP-MS. The method is capable of determining 2.5-50 mg of Zinc/kg flour, depending on instrument design. This method is not applicable to milk powder.
The food testing laboratories are instructed to start using the aforesaid method with immediate effect.
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